xenopus laevis oocytes

xenopus laevis oocytes

We have found it best to send oocytes out as dissected ovary lobes on wet ice but can provide defolliculated oocytes if necessary, albeit at extra cost. Xenopus Xenopus laevis Scientific classification Kingdom: Animalia Phylum: Chordata Class: Amphibia Order: Anura Family: Pipidae Genus: Xenopus Wagler 1827 Species See text Xenopus is a genus of highly aquatic frogs native to sub-Saharan Africa. Agents interacting with meiosis in Xenopus laevis oocytes, Some steroids: progesterone, cortisol, testosterone, pregnenolone, Some organo-mercurials: parachloromercuribenzoate, mersalyl, Some ions: lanthanum, zinc, calcium*† magnesium*, Some antiarrythmic drugs: D-600(isoptin), D-200(verapamil), Some O-receptor adrenergic antagonists: isomers of propranolol, alprenolol, dichloroisoproterenol, Some local anesthetics: dibucaine, tetracaine, Some CNS acting drugs: barbital, imipramine, chlorpromazine, fluphenazine, fenfluramine, cycloheximide (inhibitor of protein synthesis), theophylline, IBMX (inhibitors of phosphodiesterases), dithiothreitol (protecting agent for SH groups), gammexane (inhibitor of phospholipid metabolism). Bröer S(1). The same strategy was applied by different investigators to successfully clone rabbit PEPT1 and rabbit PEPT2 from the rabbit intestinal and renal cDNA libraries, respectively (29,30). The oocyte is the cellular precursor for the development of specialized cells. As King (1990) pointed out, this would require some rather unlikely chromosomal changes, making it much more parsimonious to assume that 36 chromosomes constitute a tetraploid complement. Max Birnstiel and I have reviewed the research on purified genes that preceded the recombinant DNA era. B., Lane, C. D., Woodland, H. R., et al. The relatively broad expression domain and range of phenotypes observed following its inactivation indicate that XSix3 is required not only for eye field specification, but also for much of rostral brain formation. Publications. Characteristics Cytoplasm. Interestingly, systematists now consider X. tropicalis and X. epitropicalis to warrant placement into their own genus (Silurana) on the basis of morphological characteristics (e.g., Cannatella and Trueb, 1988; de Sá and Hillis, 1990; Cannatella and de Sá, 1993; Rödel, 2000; Frost, 2004). The Xenopus oocyte, through injection of exogenous RNA, a method now more than 40 years old (Gurdon et al., 1971), is one of the major expression system for membrane proteins such as ion channels (Cens and Charnet, 2007) as well as other potential partners or regulators of the SOCE process. Xenopus laevis oocytes have been used widely in the field of electrophysiology to study the structure and the function of numerous ion channels and to screen for selective blockers. Xenopus laevis oviductin cleaves egg envelope glycoprotein gp43 to gp41 [6]. African clawed frogs of the genus Xenopus display some of the most variable ploidy levels seen among vertebrates (King, 1990). 2.4E). Xenopus are kept at 17–19°C, on a 12 hr off/12 hr on dark/light cycle. This approach effectively reduces the size of the cDNA library to be screened and increases the probability of successful isolation of the target cDNA. Oviductin is inhibited by DFP, aprotinin, soybean trypsin inhibitor, leupeptin, antipain EDTA, and EGTA, and also weakly by TLCK, TPCK, and 1,10-phenanthroline; pepstatin, E-64, and iodoacetamide do not inhibit [5]. Antimicrobial compounds (magainins) are also found in skin secretions (Kreil, 1996). Finally, a wide variety of non-hormonal compounds, applied at millimolar concentration range, were also able to trigger meiosis in Xenopus laevis oocytes (Table 1). This contributes to reducing the number of female Xenopus used for oocyte injection experiments. John P. Leonard, in Methods in Neurosciences, 1989. (2000) Swelling-induced taurine release without chloride channel activity in, Zeuthen, T., Meinild, A. K., Loo, D. D., et al. After oocyte maturation, clear white spots appear at the top of animal hemispheres and follicle cells are peeled off. Preparation. Please Specify Uncurling arrive around 38 NF

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